An in situ activity assay for lysyl oxidases

Huilei Wang; Alan Poe; Lydia Pak; Kavitha Nandakumar; Sandeep Jandu; Jochen Steppan; Reik Löser; Lakshmi Santhanam

doi:10.1038/s42003-021-02354-0

Communications Biology (Jul 2021)

An in situ activity assay for lysyl oxidases

Huilei Wang,
Alan Poe,
Lydia Pak,
Kavitha Nandakumar,
Sandeep Jandu,
Jochen Steppan,
Reik Löser,
Lakshmi Santhanam

Affiliations

Huilei Wang: Department of Biomedical Engineering, Johns Hopkins University
Alan Poe: Department of Biomedical Engineering, Johns Hopkins University
Lydia Pak: Department of Biomedical Engineering, Johns Hopkins University
Kavitha Nandakumar: Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University
Sandeep Jandu: Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University
Jochen Steppan: Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University
Reik Löser: Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf
Lakshmi Santhanam: Department of Biomedical Engineering, Johns Hopkins University

DOI: https://doi.org/10.1038/s42003-021-02354-0
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 10

Abstract

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To address the limitation of inability to detect lysyl oxidase enzymes (LOX) catalytic function associated with diverse disease processes, Wang et al. developed an in situ activity assay by utilizing the reactivity of hydrazides with imines and carbonyls. This assay detects the total LOX activity in situ for both overexpressed and endogenous LOXs in cells and tissue samples.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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