iScience (Jul 2024)
The profile of blood microbiome in new-onset type 1 diabetes children
- Xiaoxiao Yuan,
- Xin Yang,
- Zhenran Xu,
- Jie Li,
- ChengJun Sun,
- Ruimin Chen,
- Haiyan Wei,
- Linqi Chen,
- Hongwei Du,
- Guimei Li,
- Yu Yang,
- Xiaojuan Chen,
- Lanwei Cui,
- Junfen Fu,
- Jin Wu,
- Zhihong Chen,
- Xin Fang,
- Zhe Su,
- Miaoying Zhang,
- Jing Wu,
- Xin Chen,
- Jiawei Zhou,
- Yue Luo,
- Lei Zhang,
- Ruirui Wang,
- Feihong Luo
Affiliations
- Xiaoxiao Yuan
- Department of Pediatric Endocrinology and Inherited Metabolic Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China
- Xin Yang
- Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China; Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; Section of Endocrinology, Internal Medicine, School of Medicine, Yale University, New Haven, CT 06511, United States
- Zhenran Xu
- Department of Pediatric Endocrinology and Inherited Metabolic Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China
- Jie Li
- Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China; Teaching and Research Division, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong 999077, China
- ChengJun Sun
- Department of Pediatric Endocrinology and Inherited Metabolic Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China
- Ruimin Chen
- Fuzhou Children’s Hospital of Fujian Medical University, Fuzhou 350000, China
- Haiyan Wei
- Department of Endocrinology and Inherited Metabolic, Children’s Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, China
- Linqi Chen
- Children’s Hospital of Soochow University, Suzhou 215000, China
- Hongwei Du
- The First Hospital of Jilin University, Jilin 130000, China
- Guimei Li
- Department of Pediatric Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
- Yu Yang
- The Affiliated Children’s Hospital of Nanchang University, Nanchang 330006, China
- Xiaojuan Chen
- Department of Endocrinology, Genetics and Metabolism, The Children’s Hospital of Shanxi Province, Taiyuan 030013, China
- Lanwei Cui
- The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
- Junfen Fu
- Department of Endocrinology, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310005, China
- Jin Wu
- Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China
- Zhihong Chen
- Department of Neuroendocrinology Pediatrics, Affiliated Hospital of Qingdao University, Qingdao 266003, China
- Xin Fang
- Fujian Medical University Union Hospital, Fuzhou 350001, China
- Zhe Su
- Shenzhen Children’s Hospital, Shenzhen 518038, China
- Miaoying Zhang
- Department of Pediatric Endocrinology and Inherited Metabolic Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China
- Jing Wu
- Department of Pediatric Endocrinology and Inherited Metabolic Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China
- Xin Chen
- Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China
- Jiawei Zhou
- Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China
- Yue Luo
- Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
- Lei Zhang
- Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China
- Ruirui Wang
- Shanghai Innovation Center of TCM Health Service, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China; Corresponding author
- Feihong Luo
- Department of Pediatric Endocrinology and Inherited Metabolic Diseases, Children’s Hospital of Fudan University, Shanghai 201102, China; Corresponding author
- Journal volume & issue
-
Vol. 27,
no. 7
p. 110252
Abstract
Summary: Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood microbiome using 16S rRNA gene sequencing in 77 controls and 64 children with new-onset T1D, and compared it with the gut and oral microbiomes. The blood microbiome of patients with T1D is characterized by increased diversity and perturbed microbial features, with a significant increase in potentially pathogenic bacteria compared with controls. Thirty-six representative genera of blood microbiome were identified by random forest analysis, providing strong discriminatory power for T1D with an AUC of 0.82. PICRUSt analysis suggested that bacteria capable of inducing inflammation were more likely to enter the bloodstream in T1D. The overlap of the gut and oral microbiome with the blood microbiome implied potential translocation of bacteria from the gut and oral cavity to the bloodstream. Our study raised the necessity of further mechanistic investigations into the roles of blood microbiome in T1D.
