Efek Fraksi n-Heksana Kulit Hylocereus polyrhizus Terhadap Aktivitas Katalase Tikus Stres Oksidatif

Abstract

Production of excessive free radicals can cause oxidative damage and degenerative diseases. Catalase enzyme is one of the endogenous intracellular antioxidants. Its activity can be reduced by oxidative stress conditions. The peel of red dragon fruit (Hylocereus polyrhizus) as a potent exogenous antioxidant could support the internal enzyme activity to prevent free radicals. The aim of this research was to determine the effect of n-hexane fraction of H. polyrhizus’s peel and the dose that could increase the activity of catalase. The peel was maserated with chloroform, then fractionated by n-hexane. Stress condition for rats was achieved by five days fasting and swimming for 10 mins/day. UV-Vis spectrophotometer was used to measure the activity of catalase. The rats were divided into 6 groups: normal control, negative control, dose I (10 mg/200g BW), dose II (40 mg/200g BW), as well as two positive controls: quercetin (4 mg/200g BW) and vitamin E (17.64 mg/kg BW). The activity of catalase enzyme in the normal control was 29.4 units/ml; negative control was 7.08 units/ml; dose I was 14.42 units/ml; dose II was 14.79 units/ml, quercetin was 28.18 units/ml; and vitamin E was 71.29 units/ml. The conclusion of this research was that n-hexane fraction of H. polyrhizus could increase the activity of the catalase enzyme of rat under oxidative stress.

Keywords

• antioxidant
• hylocereus polyrhizus
• catalase
• antioksidan
• katalase