Stem Cell Reports (Dec 2015)
Creating Patient-Specific Neural Cells for the In Vitro Study of Brain Disorders
- Kristen J. Brennand,
- M. Carol Marchetto,
- Nissim Benvenisty,
- Oliver Brüstle,
- Allison Ebert,
- Juan Carlos Izpisua Belmonte,
- Ajamete Kaykas,
- Madeline A. Lancaster,
- Frederick J. Livesey,
- Michael J. McConnell,
- Ronald D. McKay,
- Eric M. Morrow,
- Alysson R. Muotri,
- David M. Panchision,
- Lee L. Rubin,
- Akira Sawa,
- Frank Soldner,
- Hongjun Song,
- Lorenz Studer,
- Sally Temple,
- Flora M. Vaccarino,
- Jun Wu,
- Pierre Vanderhaeghen,
- Fred H. Gage,
- Rudolf Jaenisch
Affiliations
- Kristen J. Brennand
- Department of Psychiatry, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA
- M. Carol Marchetto
- Laboratory of Genetics, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, San Diego, CA 92037, USA
- Nissim Benvenisty
- Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
- Oliver Brüstle
- Institute of Reconstructive Neurobiology, LIFE & BRAIN Center, University of Bonn, Bonn 53127, Germany
- Allison Ebert
- Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
- Juan Carlos Izpisua Belmonte
- Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, San Diego, CA 92037, USA
- Ajamete Kaykas
- Department of Neurobiology, Novartis Institute for BioMedical Research, Inc., 100 Technology Square, Cambridge, MA 02139, USA
- Madeline A. Lancaster
- Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, UK
- Frederick J. Livesey
- Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
- Michael J. McConnell
- Department of Biochemistry and Molecular Genetics, Center for Brain Immunology and Glia, University of Virginia School of Medicine, 1340 Jefferson Park Avenue, Charlottesville, VA 22908, USA
- Ronald D. McKay
- Brain Development, Lieber Institute for Brain Development, 855 North Wolfe Street, Baltimore, MD 21205, USA
- Eric M. Morrow
- Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 70 Ship Street, Providence, RI 02912, USA
- Alysson R. Muotri
- Department of Cellular & Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92037, USA
- David M. Panchision
- Developmental Neurobiology Program, National Institute of Mental Health, 6001 Executive Boulevard, Bethesda, MD 20892, USA
- Lee L. Rubin
- Department of Stem Cell and Regenerative Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
- Akira Sawa
- Department of Psychiatry and Behavioral Sciences, The Johns Hopkins Hospital, 600 North Wolfe Street, Baltimore, MD 21287, USA
- Frank Soldner
- Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
- Hongjun Song
- Institute for Cell Engineering, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA
- Lorenz Studer
- Stem Cell and Tumor Biology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 256, New York, NY 10021, USA
- Sally Temple
- Neural Stem Cell Institute, One Discovery Drive, Rensselaer, NY 12144, USA
- Flora M. Vaccarino
- Child Study Center, Yale University, 230 South Frontage Road, New Haven, CT 06520, USA
- Jun Wu
- Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, San Diego, CA 92037, USA
- Pierre Vanderhaeghen
- Institute of Interdisciplinary Research (IRIBHM), University of Brussels ULB, 808, Route de Lennik, 1070 Brussels, Belgium
- Fred H. Gage
- Laboratory of Genetics, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, San Diego, CA 92037, USA
- Rudolf Jaenisch
- Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
- DOI
- https://doi.org/10.1016/j.stemcr.2015.10.011
- Journal volume & issue
-
Vol. 5,
no. 6
pp. 933 – 945
Abstract
As a group, we met to discuss the current challenges for creating meaningful patient-specific in vitro models to study brain disorders. Although the convergence of findings between laboratories and patient cohorts provided us confidence and optimism that hiPSC-based platforms will inform future drug discovery efforts, a number of critical technical challenges remain. This opinion piece outlines our collective views on the current state of hiPSC-based disease modeling and discusses what we see to be the critical objectives that must be addressed collectively as a field.
