Protective effect of Helichrysum plicatum on head shock protein inflammation and apoptosis in Gentamicin induced nephrotoxicity

Abstract

Gentamicin (GM) is an aminoglycoside antibiotic the most common used in the treatment of infectious diseases in humans and animals. However, GM causes damage to many tissues and organs in the body, especially the kidneys. Helichrysum plicatum (Hp), native to the Balkans and Anatolia, is a plant used in various diseases such as diabetes, liver and kidney damage. In this study, Male Spraque Dawley rats (n=36 and 200–250 g) were randomly divided into 6 experimental groups: Group 1: Control; received normal saline (intraperitoneally –i.p.–), Group 2: Hp (100 mg·kg–1 day i.p.), Group 3: Hp (200 mg·kg–1 day i.p.), Group 4: GM (80 mg·kg–1 day i.p.), Group 5: GM 80 + Hp 100 (mg·kg–1 day i.p.), and Group 6: GM 80 + Hp 200 (mg·kg–1 day i.p.). Then kidney tissue samples were collected for evaluations. All of our results showed that Hp (100 mg·kg–1 day) reduced the levels of pro–inflammatory cytokines such as IL–8, IL–6, and TNF– while increasing the level of anti–inflammatory cytokine IL–10. It was also observed that Hp reduced the expressions of the caspase3, NOS and Heat shock proteins such as Hsp27 and Hsp70. With this study, we have shown that Hp probably due to its chemical properties has a protective effect against GM induced nephrototoxicity by reducing the values stated above to normal values.

Keywords

• Caspase
• gentamicin
• Helichrysum plicatum
• HSP
• kidney
• NOS