Single-cell RNA sequencing reveals epithelial cells driving brain metastasis in lung adenocarcinoma

Yonghui Wu; Fujun Yang; Shilan Luo; Xiang Li; Zhan Gu; Rui Fan; Yajuan Cao; Lixin Wang; Xiao Song

iScience (Mar 2024)

Single-cell RNA sequencing reveals epithelial cells driving brain metastasis in lung adenocarcinoma

Yonghui Wu,
Fujun Yang,
Shilan Luo,
Xiang Li,
Zhan Gu,
Rui Fan,
Yajuan Cao,
Lixin Wang,
Xiao Song

Affiliations

Yonghui Wu: Department of Integrated Traditional Chinese and Western Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China; Graduate School of Shanghai University of Traditional Chinese Medicine, Shanghai, China
Fujun Yang: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Shilan Luo: Department of Radiation Oncology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Xiang Li: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Zhan Gu: Department of Integrated Traditional Chinese and Western Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Rui Fan: Department of Integrated Traditional Chinese and Western Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Yajuan Cao: Department of Integrated Traditional Chinese and Western Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China; Corresponding author
Lixin Wang: Department of Integrated Traditional Chinese and Western Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China; Corresponding author
Xiao Song: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China; Corresponding author

Journal volume & issue: Vol. 27, no. 3
p. 109258

Abstract

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Summary: Brain metastases (BM) of lung adenocarcinoma (LUAD) are the most common intracranial malignancy leading to death. However, the cellular origins and drivers of BM from LUAD have not been clarified. Cellular composition was characterized by single-cell sequencing analysis of primary lung adenocarcinoma (pLUAD), BM and lymph node metastasis (LNM) samples in GSE131907. Our study briefly analyzed the tumor microenvironment (TME), focusing on the role of epithelial cells (ECs) in BM. We have discovered a population of brain metastasis-associated epithelial cells (BMAECs) expressing SPP1, SAA1, and CDKN2A, and it has been observed that this population is mainly composed of aneuploid cells from pLUAD, playing a crucial role in brain metastasis. Our study concluded that both LNM and BM in LUAD originated from pLUAD lesions, but there is currently insufficient evidence to prove a direct association between BM lesions and LNM lesions, which provides inspiration for further investigation of the TME in BM.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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