Effect of two plant growth regulators and illumination conditions in the germination of conserved seeds of<i> Clitoria ternatea</i>

Abstract

The seeds viability lost in the seed legume bank of Research Institute of Pastures and Forages (IIPF) led to the aim of the work it was to determine the effect of two plant growth regulators (gibberellic acid; GA3 and naphthalene acetic acid; ANA) and illumination conditions on the germination of Clitoria ternatea SC-136 conserved seeds. One experiment was performed with two-factor completely randomized design with four replications Five different levels of growth regulators (factor A) and two illumination conditions (factor B) were evaluated. The variables measured were: total germination percentage (PTG) and angular transformation, days to 50% PTG (G50) and the days between 10 and 90% PTG (G10-90). In addition, morphological variables were evaluated. It was found that the addition of plant growth regulators (GA3 and NAA) was effective in increasing germination of Clitoria ternatea SC-136 conserved seeds, but not the illumination conditions tested. Combination GA3 (1 mg l-1) and NAA (0.1 mg l-1) to stimulate germination was recommended. Key words: GA3, germplasm, legume, NAA, photoperiod.