Nature Communications (Apr 2021)
CRISPR-based genome editing in primary human pancreatic islet cells
- Romina J. Bevacqua,
- Xiaoqing Dai,
- Jonathan Y. Lam,
- Xueying Gu,
- Mollie S. H. Friedlander,
- Krissie Tellez,
- Irene Miguel-Escalada,
- Silvia Bonàs-Guarch,
- Goutham Atla,
- Weichen Zhao,
- Seung Hyun Kim,
- Antonia A. Dominguez,
- Lei S. Qi,
- Jorge Ferrer,
- Patrick E. MacDonald,
- Seung K. Kim
Affiliations
- Romina J. Bevacqua
- Department of Developmental Biology, Stanford University School of Medicine
- Xiaoqing Dai
- Alberta Diabetes Institute and Department of Pharmacology, University of Alberta
- Jonathan Y. Lam
- Department of Developmental Biology, Stanford University School of Medicine
- Xueying Gu
- Department of Developmental Biology, Stanford University School of Medicine
- Mollie S. H. Friedlander
- Department of Developmental Biology, Stanford University School of Medicine
- Krissie Tellez
- Department of Developmental Biology, Stanford University School of Medicine
- Irene Miguel-Escalada
- Bioinformatics and Genomics Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Silvia Bonàs-Guarch
- Bioinformatics and Genomics Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Goutham Atla
- Bioinformatics and Genomics Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Weichen Zhao
- Department of Developmental Biology, Stanford University School of Medicine
- Seung Hyun Kim
- Department of Developmental Biology, Stanford University School of Medicine
- Antonia A. Dominguez
- Department of Bioengineering, Stanford University
- Lei S. Qi
- Department of Bioengineering, Stanford University
- Jorge Ferrer
- Bioinformatics and Genomics Program, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)
- Patrick E. MacDonald
- Alberta Diabetes Institute and Department of Pharmacology, University of Alberta
- Seung K. Kim
- Department of Developmental Biology, Stanford University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-021-22651-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
The editing of primary human islets could provide insight into diabetes pathogenesis. Here the authors use CRISPR-Cas9 to target regulatory elements associated with T2D susceptibility.
