Chromosome genome assembly and annotation of Adzuki Bean (Vigna angularis)

Abstract

Abstract Adzuki bean (Vigna angularis) is a significant dietary legume crop that is prevalent in East Asia. It also holds traditional medicinal importance in China. In this study, we report a high-quality, chromosome-level genome assembly of adzuki bean obtained by employing Illumina short-read sequencing, PacBio long-read sequencing, and Hi-C technology. The assembly spans 447.8 Mb, encompassing 96.32% of the estimated genome, with contig and scaffold N50 values of 16.5 and 41.0 Mb, respectively. More than 98.2% of the 1,614 BUSCO genes were fully identified, and 25,939 genes were annotated, with 98.23% of them being functionally identifiable. Vigna angularis was estimated to diverge successively from Vigna unguiculata and Vigna radiata about 15.3 and 8.7 million years ago (Ma), respectively. This chromosome-level reference genome of Vigna angularis provides a robust foundation for exploring the functional genomics and genome evolution of adzuki bean, thereby facilitating advancements in molecular breeding of adzuki bean.