Characteristics of T2* and anisotropy parameters in inguinal and epididymal adipose tissues after cold exposure in mice

Abstract

Abstract White adipose tissue (WAT) in mice undergoes browning in response to cold exposure. Brown and beige adipocytes contain multilocular lipid droplets and abundant iron-containing mitochondria expressing uncoupling protein 1 (UCP-1). Cold exposure-induced browning WAT is accompanied by increased density of blood vessels and sympathetic nerve fibres. A previous study reported a more than threefold increase in sympathetic nerve dendritic tone in inguinal white adipose tissue (iWAT) after cold exposure. Therefore, we hypothesized that water molecule diffusion would be more restricted in brown and beige adipocytes compared to white adipocytes. The characteristics of T2* values and anisotropy parameters by diffusion tensor imaging (DTI) in browning WAT are unclear. The aim of the present study was to investigate the effect of cold exposure on T2* values and anisotropy parameters (fractional anisotropy [FA], apparent diffusion coefficient [ADC], radial diffusivity [RD] and eigenvalues λ1, λ2, λ3) in brown adipose tissue (BAT), iWAT and epididymal white adipose tissue (epiWAT). Furthermore, these parameters were investigated in vivo through additional validation experiments in three control mice. Mice in the cold exposure (CE) group were exposed to a cold environment at 4 °C for 10 days, while these in the control (C) group were maintained at 22 °C throughout the experiment. T2* values, FA, ADC, RD and eigenvalues (λ1, λ2, λ3) were measured in BAT, iWAT and epiWAT using a 9.4T magnetic resonance scanner (Bruker Biospin AG). T2* values of epiWAT in the C group were significantly higher than these of BAT in the C group and iWAT in the CE group. No significant differences were observed between groups for FA, ADC, RD, λ1 and λ2 of iWAT and epiWAT. However, the λ3 values of iWAT and epiWAT in the CE group were significantly higher than these of iWAT, epiWAT and BAT in the C group. Compared to ex vivo measurements, in vivo measurements in control mice showed higher T2* values with reduced intertissue variability while maintaining tissue-specific patterns. These results suggest that T2* values and anisotropy parameters might serve as potential markers for the assessment of adipose tissue plasticity. Further studies are required to investigate their utility as non-invasive indicators of browning WAT.

Keywords

• Browning
• White adipose tissue
• Brown adipose tissue
• Diffusion tensor imaging
• T2* mapping