Improved separation of sterols by reversed-phase thin-layer chromatography

Abstract

Some closely related sterols have been separated with better resolution in a shorter period of time than has previously been reported. Separations were effected on the basis of carbon number and the number and location of double bonds through the use of paraffin-impregnated kieselguhr chromatoplates in the system (paraffin oil)/(acetone-water 4:1).

Keywords

• phytosterols
• cholesterol precursors