Anti-Tumour Promoting Activity and Antioxidant Properties of Girinimbine Isolated from the Stem Bark of<em> Murraya koenigii </em>S.

Abstract

Girinimbine, a carbazole alkaloid isolated from the stem bark of <em>Murraya koenigii </em>was tested for the<em> in vitro</em> anti-tumour promoting and antioxidant activities. Anti-tumour promoting activity was determined by assaying the capability of this compound to inhibit the expression of early antigen of Epstein-Barr virus (EA-EBV) in Raji cells that was induced by the tumour promoter, phorbol 12-myristate 13-acetate. The concentration of this compound that gave an inhibition rate at fifty percent was 6.0 µg/mL and was not cytotoxic to the cells. Immunoblotting analysis of the expression of EA-EBV showed that girinimbine was able to suppress restricted early antigen (EA-R). However, diffused early antigen (EA-D) was partially suppressed when used at 32.0 µg/mL. Girinimbine exhibited a very strong antioxidant activity as compared to a-tocopherol and was able to inhibit superoxide generation in the 12-<em>O</em>-tetradecanoylphorbol-13-acetate (TPA)-induced differentiated premyelocytic HL-60 cells more than 95%, when treated with the compound at 5.3 and 26.3 µg/mL, respectively<strong>.</strong> However girinimbine failed to scavenge the stable diphenyl picryl hydrazyl (DPPH)-free radical.

Keywords

• &lt;em&gt;Murraya&lt;/em&gt; &lt;em&gt;koenigii&lt;/em&gt;
• girinimbine
• anti-tumour promoting activity
• antioxidative
• superoxide