Studying the Association of Gene Expressions and Gene Polymorphisms of MAPK14 (rs28763973) and HTR2B (rs550352538) with Gastric Cancer in Isfahan Province

Abstract

Objective: Gastric cancer (GC) is the fourth most common cancer worldwide, occurring in approximately two-thirds of developing countries, and is one of the leading causes of cancer deaths. The mitogen-activated protein kinase 14 (MAPK14) plays an important role in the conversion of extracellular stimuli to a wide range of cellular responses. The 5-hydroxytryptamine receptor 2B (HTR2B) increases the level of 5-HT; and as a result, platelet-derived 5-HT promotes tumor angiogenesis, tumor growth, and metastatic potential of cancer cells. Materials and Methods: Expression analysis was performed on 20 healthy cases and 20 cancerous cases for MAPK14 and 20 healthy cases and 19 cancerous cases for HTR2B using real-time quantitative PCR (qRT-PCR) assay. Polymorphisms were evaluated in 38 healthy cases and 30 cancerous cases for rs550352538, and 35 healthy cases and 35 cancerous cases for rs28763973 via tetra-primer amplification refractory mutation system PCR (T-ARMS PCR) technique. Results: No significant difference was observed in MAPK14 and HTR2B gene expression levels (P>0.05). In addition, there is no statistically significant association of different genotypes in rs28763973 from MAPK14 gene and rs550352538 from HTR2B gene with gastric cancer (P>0.05). Conclusions: No significant difference was noted between patient and healthy individuals in expression levels and gene polymorphisms; nevertheless, results may vary by significantly changing the gene pool or population size.

Keywords

• stomach neoplasms
• real-time polymerase chain reaction
• genetic variation
• mitogen-activated protein kinase 14
• receptor, serotonin, 5-ht2b