Electrophysiologic and anti‐inflammatorial effects of cyclooxygenase inhibition in epileptiform activity

Abstract

Abstract The aim of our study is to investigate the electrophysiological and anti‐inflammatory effects of diclofenac potassium on epileptiform activity, which is the liquid form of diclofenac, and frequently used clinically for inflammatory process by inhibiting cyclooxygenase enzyme (COX). Wistar rats aged 2–4 months were divided into Epilepsy, Diazepam, Diclofenac potassium, and Diazepam+diclofenac potassium groups. Diazepam and diclofenac potassium were administered intraperitoneally 30 min after the epileptiform activity was created with penicillin injected intracortically under anesthesia. After the electrophysiological recording was taken in the cortex for 125 min, interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) were evaluated by the ELISA in the serums. No change was observed between the groups in serum IL‐1β, IL‐6, and TNF‐α values. It was observed that the co‐administration of diclofenac potassium and diazepam at 51–55, 56–60, 61–65, 111–115, and 116–120 min was more effective in reducing spike amplitude than diclofenac potassium alone (p < 0.05). Single‐dose diclofenac potassium did not have an anti‐inflammatory effect in epileptiform activity but both diazepam and diclofenac potassium reduced the epileptiform activity.

Keywords

• acute seizure
• anti‐inflammatory
• diclofenac potassium
• electrophysiology
• penicillin‐induced epileptiform activity