Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> Mosquitoes from Brazil

Abstract

Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.

Keywords

• Hubei reo-like virus 7
• reovirus
• RNA-dependent RNA polymerase
• metagenomic
• <i>Aedes aegypti</i>
• <i>Culex quinquefasciatus</i>
• mosquitoes
• insect-viruses
• Amazon forest
• arbovirus
• birds
• Brazil