Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs

Takashi Ikeda; Takafusa Hikichi; Hisashi Miura; Hirofumi Shibata; Kanae Mitsunaga; Yosuke Yamada; Knut Woltjen; Kei Miyamoto; Ichiro Hiratani; Yasuhiro Yamada; Akitsu Hotta; Takuya Yamamoto; Keisuke Okita; Shinji Masui

doi:10.1038/s41467-018-03748-1

Nature Communications (Apr 2018)

Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs

Takashi Ikeda,
Takafusa Hikichi,
Hisashi Miura,
Hirofumi Shibata,
Kanae Mitsunaga,
Yosuke Yamada,
Knut Woltjen,
Kei Miyamoto,
Ichiro Hiratani,
Yasuhiro Yamada,
Akitsu Hotta,
Takuya Yamamoto,
Keisuke Okita,
Shinji Masui

Affiliations

Takashi Ikeda: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Takafusa Hikichi: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Hisashi Miura: RIKEN Center for Developmental Biology (CDB)
Hirofumi Shibata: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Kanae Mitsunaga: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Yosuke Yamada: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Knut Woltjen: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Kei Miyamoto: Faculty of Biology-Oriented Science and Technology, Laboratory of Molecular Developmental Biology, Kindai University
Ichiro Hiratani: RIKEN Center for Developmental Biology (CDB)
Yasuhiro Yamada: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Akitsu Hotta: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Takuya Yamamoto: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Keisuke Okita: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
Shinji Masui: Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University

DOI: https://doi.org/10.1038/s41467-018-03748-1
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 15

Abstract

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The transcription factor Srf is a central regulator of immediate-early and actin cytoskeletal genes. Here the authors show that Srf is activated by repression of β-actin, promoting iPSC reprogramming of neural progenitor cells and hepatoblasts by repressing cell-type specific genes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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