Screening of brucellosis in goats by RBPT and c-Elisa in organized farms in Chennai, India

Abstract

The prevalence of brucellosis among goats was studied in organized farms in and around Chennai, Tamil Nadu, by conventional Rose Bengal Plate Test (RBPT) and also with c-ELISA using Bru Alert® Brucella antibody kit. In a total of 161 sera samples screened for the presence of Brucella antibody, the overall positivities of 4.97% and 5.59 % were respectively found by RBPT and c-ELSA. The study also recorded high seropositivity in animals past 5 years of age and in female animals. A comparative analysis showed excellent agreement of the two tests and the chi-square test indicated strong (P<0.01) association between the alternative tests. The study arrived at the conclusion that initial screening for brucellosis could be done with RBPT and confirmation should be made with c- ELISA test to eliminate false positives.

Keywords

• abortion
• organized farm
• caprine brucellosis
• c-elisa
• rbpt