Frontiers in Immunology (Jan 2023)
Duck TRIM29 negatively regulates type I IFN production by targeting MAVS
- Weiqiang Li,
- Weiqiang Li,
- Weiqiang Li,
- Yating Song,
- Yating Song,
- Yating Song,
- Yuqing Du,
- Yuqing Du,
- Yuqing Du,
- Zhanhong Huang,
- Zhanhong Huang,
- Zhanhong Huang,
- Meng Zhang,
- Meng Zhang,
- Meng Zhang,
- Zuxian Chen,
- Zuxian Chen,
- Zuxian Chen,
- Zhuoliang He,
- Zhuoliang He,
- Zhuoliang He,
- Yangbao Ding,
- Yangbao Ding,
- Yangbao Ding,
- Junsheng Zhang,
- Junsheng Zhang,
- Junsheng Zhang,
- Luxiang Zhao,
- Luxiang Zhao,
- Luxiang Zhao,
- Hailiang Sun,
- Hailiang Sun,
- Hailiang Sun,
- Peirong Jiao,
- Peirong Jiao,
- Peirong Jiao
Affiliations
- Weiqiang Li
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Weiqiang Li
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Weiqiang Li
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Yating Song
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yating Song
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Yating Song
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Yuqing Du
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yuqing Du
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Yuqing Du
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Zhanhong Huang
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhanhong Huang
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Zhanhong Huang
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Meng Zhang
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Meng Zhang
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Meng Zhang
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Zuxian Chen
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zuxian Chen
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Zuxian Chen
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Zhuoliang He
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhuoliang He
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Zhuoliang He
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Yangbao Ding
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yangbao Ding
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Yangbao Ding
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Junsheng Zhang
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Junsheng Zhang
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Junsheng Zhang
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Luxiang Zhao
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Luxiang Zhao
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Luxiang Zhao
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Hailiang Sun
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Hailiang Sun
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Hailiang Sun
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- Peirong Jiao
- Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Peirong Jiao
- Key Laboratory of Animal Vaccine Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China
- Peirong Jiao
- Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, China
- DOI
- https://doi.org/10.3389/fimmu.2022.1016214
- Journal volume & issue
-
Vol. 13
Abstract
The innate immune response is a host defense mechanism that induces type I interferon and proinflammatory cytokines. Tripartite motif (TRIM) family proteins have recently emerged as pivotal regulators of type I interferon production in mammals. Here, we first identified duck TRIM29, which encodes 571 amino acids and shows high sequence homology with other bird TRIM29 proteins. DuTRIM29 inhibited IFN-β and IRF7 promoter activation in a dose-dependent manner and downregulated the mRNA expression of IFN-β, IRF7, Mx and IL-6 mediated by duRIG-I. Moreover, duTRIM29 interacted and colocalized with duMAVS in the cytoplasm. DuTRIM29 interacted with duMAVS via its C-terminal domains. In addition, duTRIM29 inhibited IFN-β and IRF7 promoter activation and significantly downregulated IFN-β and immune-related gene expression mediated by duMAVS in ducks. Furthermore, duTRIM29 induced K29-linked polyubiquitination and degradation of duMAVS to suppress the expression of IFN-β. Overall, our results demonstrate that duTRIM29 negatively regulates type I IFN production by targeting duMAVS in ducks. This study will contribute to a better understanding of the molecular mechanism regulating the innate immune response by TRIM proteins in ducks.
Keywords
