OptoGenie: an open-source device for the optogenetic stimulation of cells

Abstract

Optogenetics has revolutionised research in cell biology over the past 15 years, yet devices that can effectively stimulate cells using light are often costly and specifically designed for a single experimental set-up with little flexibility. Our novel ‘OptoGenie’ stimulation device can be conveniently transferred between cell culture incubators for long-term stimulation, electrophysiology rigs for patch-clamp recordings, and optical microscopes for fluorescence imaging of cells. The modular design of the device offers portability between these experimental set-ups, is low cost compared with commercial devices, and provides easy adjustment of the stimulation intensity and frequency. OptoGenie provides an open-source model made from proprietary parts such that researchers without experience with electronics and coding can easily purchase, assemble and customise for their experimental needs.

Keywords

• optogenetics
• neuroscience
• microscopy
• arduino
• electrophysiology