Drug Design, Development and Therapy (Dec 2020)
Modulation of Drug Release from Natural Polymer Matrices by Response Surface Methodology: in vitro and in vivo Evaluation
Abstract
Afrasim Moin,1 Hosahalli V Gangadharappa,2 Mohd Adnan,3 Syed M Rizvi,1 Syed A Ashraf,4 Mitesh Patel,5 Amr S Abu Lila,1,6 Ahmed N Allam7 1Department of Pharmaceutics, College of Pharmacy, University of Hail, Hail, Saudi Arabia; 2Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, India; 3Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia; 4Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, Hail, Saudi Arabia; 5Bapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, Surat, Gujarat, India; 6Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt; 7Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, EgyptCorrespondence: Ahmed N AllamDepartment of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria 21521, EgyptTel +20 100 5422491Email [email protected]: The present work aimed at challenging the efficacy of natural gums, karaya and locust bean gum, as matrix-forming polymers for the formulation of sustained-release tablets of diltiazem, a model drug.Methods: Central design composite was adopted for the formulation and optimization of tablet formulations. The two gums have been selected as independent variables. The dependent factors chosen were the amount of drug released in 1st hour (Y1), amount of drug released after 12 h (Y2), diffusion exponent (Y3), and time for half of the total drug released (T50%) (Y4). Wet granulation approach was used for the formulation of tablets. FT-IR, DSC, in vitro dissolution, swelling-erosion investigations, SEM, and stability studies were carried out.Results and Discussion: It was evident that the release pattern from the prepared formulations was significantly influenced by the quantity of gum(s) in the tablet. FT-IR and DSC results confirm drug–polymer compatibility. Polynomial equations were used for the prediction of quantitative impact of independent factors at different levels on response variables. After ANOVA analysis, the significant factors were considered for constrained optimization to get the optimized formula. The optimized formula generated by the response surface methodology was evaluated both for in vitro and in vivo properties. The optimized formula and a sustained-release marketed product were subjected to in vivo studies in rabbits and the results of the t-test demonstrated insignificant variation in pharmacokinetic parameters among the two formulations, confirming that the prepared tablet showed sustained-release profile.Conclusion: The results indicated that karaya and locust bean gum can be effectively used to formulate sustained-release tablets.Keywords: locust bean gum, karaya gum, diltiazem hydrochloride, response surface methodology, sustained release
