Chitosan modified graphite electrodes developed for electrochemical monitoring of interaction between daunorubicin and DNA

Abstract

Chitosan (CHIT) modified single-use electrodes were developed for the first time in the present study and applied for electrochemical monitoring of anticancer drug-DNA interaction. Under this aim, pencil graphite electrode (PGE) was used as the biosensor platform and the modification of PGEs using biopolymer, CHIT was performed by passive adsorption process. Microscopic and electrochemical characterizations of CHIT modified PGEs were performed. An anticancer drug, Daunorubicin (DNR) was analyzed by using both PGEs and CHIT-PGEs. Moreover, the effect of CHIT modification on biosensor development upon to the sensitivity of voltammetric detection of DNR and DNA was also investigated. The interaction between DNA and DNR was then performed and accordingly, the voltammetric measurements were performed before/after interaction process by using differential pulse voltammetry (DPV) technique. Keywords: Biomaterial modified biosensor, Anticancer drug-DNA interaction, Chitosan modified electrode, Daunorubicin, Differential pulse voltammetry