Chemical reversible crosslinking enables measurement of RNA 3D distances and alternative conformations in cells

Ryan Van Damme; Kongpan Li; Minjie Zhang; Jianhui Bai; Wilson H. Lee; Joseph D. Yesselman; Zhipeng Lu; Willem A. Velema

doi:10.1038/s41467-022-28602-3

Nature Communications (Feb 2022)

Chemical reversible crosslinking enables measurement of RNA 3D distances and alternative conformations in cells

Ryan Van Damme,
Kongpan Li,
Minjie Zhang,
Jianhui Bai,
Wilson H. Lee,
Joseph D. Yesselman,
Zhipeng Lu,
Willem A. Velema

Affiliations

Ryan Van Damme: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
Kongpan Li: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
Minjie Zhang: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
Jianhui Bai: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
Wilson H. Lee: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
Joseph D. Yesselman: Department of Chemistry, University of Nebraska-Lincoln
Zhipeng Lu: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
Willem A. Velema: Institute for Molecules and Materials, Radboud University Nijmegen

DOI: https://doi.org/10.1038/s41467-022-28602-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Determination of RNA 3D structures in vivo is a challenging problem. Here, the authors describe a chemical crosslinking method (SHARC) that they use in combination with RNA sequencing to measure distances between nucleotides in RNA 3D structures.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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