Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive

Stephanie Gamez; Duverney Chaverra-Rodriguez; Anna Buchman; Nikolay P. Kandul; Stelia C. Mendez-Sanchez; Jared B. Bennett; Héctor M. Sánchez C.; Ting Yang; Igor Antoshechkin; Jonny E. Duque; Philippos A. Papathanos; John M. Marshall; Omar S. Akbari

doi:10.1038/s41467-021-27333-1

Nature Communications (Dec 2021)

Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive

Stephanie Gamez,
Duverney Chaverra-Rodriguez,
Anna Buchman,
Nikolay P. Kandul,
Stelia C. Mendez-Sanchez,
Jared B. Bennett,
Héctor M. Sánchez C.,
Ting Yang,
Igor Antoshechkin,
Jonny E. Duque,
Philippos A. Papathanos,
John M. Marshall,
Omar S. Akbari

Affiliations

Stephanie Gamez: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Duverney Chaverra-Rodriguez: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Anna Buchman: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Nikolay P. Kandul: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Stelia C. Mendez-Sanchez: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Jared B. Bennett: Biophysics Graduate Group, University of California
Héctor M. Sánchez C.: Divisions of Epidemiology & Biostatistics, School of Public Health, University of California
Ting Yang: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Igor Antoshechkin: Division of Biology and Biological Engineering (BBE), California Institute of Technology
Jonny E. Duque: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego
Philippos A. Papathanos: Department of Entomology, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem
John M. Marshall: Divisions of Epidemiology & Biostatistics, School of Public Health, University of California
Omar S. Akbari: Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego

DOI: https://doi.org/10.1038/s41467-021-27333-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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CRISPR-based engineering can be used to bias sex ratios. Here the authors develop a transgenic line of Drosophila melanogaster expressing Cas9 from the Y chromosome and functionally characterize the utility of this strain for both sex selection and gene drive.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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