Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin

Ching-Yi Yiu; Shih-Ying Chen; Tsai-Hsiu Yang; Che-Jung Chang; Dong-Bor Yeh; Yi-Jie Chen; Tsuey-Pin Lin

doi:10.3390/molecules19011258

Molecules (Jan 2014)

Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin

Ching-Yi Yiu,
Shih-Ying Chen,
Tsai-Hsiu Yang,
Che-Jung Chang,
Dong-Bor Yeh,
Yi-Jie Chen,
Tsuey-Pin Lin

Affiliations

Ching-Yi Yiu: Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan
Shih-Ying Chen: Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, Taiwan
Tsai-Hsiu Yang: Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, Taiwan
Che-Jung Chang: Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan
Dong-Bor Yeh: Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan
Yi-Jie Chen: Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, Taiwan
Tsuey-Pin Lin: Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, Taiwan

DOI: https://doi.org/10.3390/molecules19011258
Journal volume & issue: Vol. 19, no. 1
pp. 1258 – 1272

Abstract

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Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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