Frontiers in Pharmacology (Jul 2022)
Worenine Prevents Solar Ultraviolet–Induced Sunburn by Inhibiting JNK2
- Juanjuan Xiao,
- Juanjuan Xiao,
- Hui Lu,
- Hui Lu,
- Tengfei Ma,
- Xiaofang Ni,
- Teding Chang,
- Man Liu,
- Nijie Li,
- Peijiang Lu,
- Changshu Ke,
- Qin Tian,
- Ling Zou,
- Fei Wang,
- Wei Wang,
- Lu Zhang,
- Ping Yuan,
- Lin Liu,
- Jianmin Zhang,
- Fei Shi,
- Qiuhong Duan,
- Feng Zhu,
- Feng Zhu,
- Feng Zhu,
- Feng Zhu
Affiliations
- Juanjuan Xiao
- Cancer Research Institute, The Affiliated Hospital of Guilin Medical University, Guilin, China
- Juanjuan Xiao
- Guangxi Health Commission Key Laboratory of Novel Onco-Kinases in Target Therapy, The Affiliated Hospital of Guilin Medical University, Guilin, China
- Hui Lu
- Wuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Hui Lu
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Tengfei Ma
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Xiaofang Ni
- Wuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Teding Chang
- Second Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Man Liu
- Second Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Nijie Li
- Second Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Peijiang Lu
- Second Clinical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Changshu Ke
- Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Qin Tian
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Ling Zou
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Fei Wang
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Wei Wang
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Lu Zhang
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Ping Yuan
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Lin Liu
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Jianmin Zhang
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Fei Shi
- Department of Dermatology, The General Hospital of Air Force, Beijing, China
- Qiuhong Duan
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Feng Zhu
- Cancer Research Institute, The Affiliated Hospital of Guilin Medical University, Guilin, China
- Feng Zhu
- Guangxi Health Commission Key Laboratory of Novel Onco-Kinases in Target Therapy, The Affiliated Hospital of Guilin Medical University, Guilin, China
- Feng Zhu
- Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Feng Zhu
- Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, The Affiliated Hospital of Guilin Medical University, Guilin, China
- DOI
- https://doi.org/10.3389/fphar.2022.881042
- Journal volume & issue
-
Vol. 13
Abstract
Excessive solar ultraviolet (SUV) radiation often causes dermatitis, photoaging, and even skin cancer. In the pathological processes of SUV-induced sunburn, JNK is activated by phosphorylation, and it in turn phosphorylates its downstream transcription factors, such as ATF2 and c-jun. The transcription factors further regulate the expression of pro-inflammatory genes, such as IL-6 and TNF-α, which ultimately leads to dermatitis. Therefore, inhibiting JNK may be a strategy to prevent dermatitis. In this study, we screened for worenine as a potential drug candidate for inhibiting sunburn. We determined that worenine inhibited the JNK-ATF2/c-jun signaling pathway and the secretion of IL-6 and TNF-α in cell culture and in vivo, confirming the role of worenine in inhibiting sunburn. Furthermore, we determined that worenine bound and inhibited JNK2 activity in vitro through the MST, kinase, and in vitro kinase assays. Therefore, worenine might be a promising drug candidate for the prevention and treatment of SUV-induced sunburn.
Keywords
