Química Nova (Jan 2012)

Desenvolvimento e validação de método analítico para quantificação do fármaco bevacizumabe por cromatografia a líquido de alta eficiência

  • Elionai Cassiana de Lima Gomes,
  • Armando da Silva Cunha Júnior,
  • Maria Irene Yoshida,
  • Rodrigo Jorge

DOI
https://doi.org/10.1590/S0100-40422012000300029
Journal volume & issue
Vol. 35, no. 3
pp. 608 – 611

Abstract

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In this study, an analytical method was developed and validated for quantitation of the drug bevacizumab (Avastin®) by high performance liquid chromatography (HPLC). The HPLC column was a BioSuite 250® HR SEC, 300 x 7.8 mm x 5 µm (Waters, USA). The mobile phase consisted of phosphate buffered saline (PBS). The results revealed that the method was specific, precise, accurate, robust and linear (r² = 0.998) from 5 to 75 µg mL-1. Therefore, this method can be used in drug release studies or in quality control ampoules of the drug.

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