Communications Biology (Nov 2022)
Cell-free synthesis of amyloid fibrils with infectious properties and amenable to sub-milligram magic-angle spinning NMR analysis
- Alons Lends,
- Asen Daskalov,
- Ansis Maleckis,
- Aline Delamare,
- Mélanie Berbon,
- Axelle Grélard,
- Estelle Morvan,
- Jayakrishna Shenoy,
- Antoine Dutour,
- James Tolchard,
- Abdelmajid Noubhani,
- Marie-France Giraud,
- Corinne Sanchez,
- Birgit Habenstein,
- Gilles Guichard,
- Guillaume Compain,
- Kristaps Jaudzems,
- Sven J. Saupe,
- Antoine Loquet
Affiliations
- Alons Lends
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Asen Daskalov
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Ansis Maleckis
- Latvian Institute of Organic Synthesis
- Aline Delamare
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Mélanie Berbon
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Axelle Grélard
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Estelle Morvan
- Univ. Bordeaux, CNRS, INSERM, IECB, UAR
- Jayakrishna Shenoy
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Antoine Dutour
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- James Tolchard
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Abdelmajid Noubhani
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Marie-France Giraud
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Corinne Sanchez
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Birgit Habenstein
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Gilles Guichard
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Guillaume Compain
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- Kristaps Jaudzems
- Latvian Institute of Organic Synthesis
- Sven J. Saupe
- Univ. Bordeaux, CNRS, IBGC, UMR 5095
- Antoine Loquet
- Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB
- DOI
- https://doi.org/10.1038/s42003-022-04175-1
- Journal volume & issue
-
Vol. 5,
no. 1
pp. 1 – 10
Abstract
A method for cell-free synthesis of amyloid fibrils is reported that also allows for solid-state NMR analysis to characterize complex and pathological fibrillary assemblies.
