Micropropagation of basil ‘Grecco a Palla’ mediated by 6-benzylaminopurine and 3-indole butyric acid

Abstract

ABSTRACT: The present study evaluated the efficiency of a protocol for micropropagation of stem apexes and nodal segments of basil ‘Grecco a Palla’ in various concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA). A completely randomized design was used with six treatments distributed in five replications. A medium without growth regulators favored the survival of Ocimumbasilicum stem apexes inoculated in vitro, and thereby promoted the sprouting of explants, whereas, for nodal segments, it was necessary to use regulators, and the concentration of 0.5 mg.L−1 BAP 0.0 mg.L−1 of IBA was more beneficial for the species.

Keywords

• auxin
• cytokinin
• in vitro culture
• Lamiaceae
• Ocimum basilicum L