Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells

Abstract

Objective To investigate the effect of actin binding protein ANLN on the proliferation, apoptosis, migration and invasion of gastric cancer cells in vitro. Methods RT-PCR and Western blot were used to detect the expression of ANLN in gastric cancer cell lines and gastric mucosal cell lines and verify the knockdown of ANLN gene by siRNA. The effect of silenced ANLN gene expression on the migration and invasion abilities of gastric cancer cells was detected by scratch test and Transwell assay. CCK-8 assay, cell fluorescence staining and flow cytometry were used to detect the effects of silent ANLN gene expression on the proliferation and apoptosis of gastric cancer cells. Results The expression of ANLN mRNA and protein in gastric cancer cell line SGC-7901/MGC-803 were significantly higher than those in gastric mucosa cell line GES-1 (P < 0.001). The transfection was successful, and the mRNA and protein expressions in the transfection group were statistically different from those in the control group (P < 0.05). Down-regulating ANLN expression significantly reduced the migration, invasion, proliferation and apoptosis of gastric cancer cells. Conclusion Down-regulating ANLN expression could significantly reduce the infiltration, migration, proliferation and apoptosis of gastric cancer cells.

Keywords

• anln
• gastric cancer
• apoptosis
• migration
• proliferation