Guangdong nongye kexue (May 2023)
Effects of Different LED Light Quality Ratio and Light Intensity on Tissue Culture of A New Anthurium andraeanum Cultivar Fuxing
Abstract
【Objective】The light condition is one of the key factors that affect the tissue culture efficiency of Anthurium andraeanum. The effects of different LED light quality ratio and light intensity on different stages of A. andraeanum tissue culture were studied to explore these light conditions suitable for each stage of tissue culture of A. andraeanum and provide an important technical reference for efficient tissue culture of A. andraeanum.【Method】The new A. andraeanum cultivar Fuxing was used as the experimental material, and the effects of different proportions of red and blue LED light ratio and light intensity on callus induction, proliferation culture and strong seedling culture of A. andraeanum Fuxing were analyzed.【Result】The LED red light quality was more suitable for the callus induction of A. andraeanum Fuxing, the 10% R light quality condition with light intensity of 100 lx had the highest callus induction rate of 79.58%, and the callus mass was yellow-green, compact, large in volume and good in state, which was suitable for the callus induction of A. andraeanum Fuxing leaves. The light quality ratio of R ∶ B = 3 ∶ 1 was suitable for callus proliferation culture of A. andraeanum Fuxing, and the proliferation coefficient was the highest (6.44) when the light intensity was 600 lx. Compared with WL light conditions, R ∶ B = 4 ∶ 1 light quality ratio was more suitable for the callus bud differentiation culture of A. andraeanum Fuxing, when the light intensity was 1 000 lx, the number of callus proliferation and differentiation was 19.44, and the buds were short and strong with good growth potential, without yellow leaf phenomenon. The light quality ratio suitable for the tissue culture seedling culture of A. andraeanum Fuxing at the strong seedling stage was R∶B = 4∶1, the light intensity was 900 lx, the number of roots of the strong seedlings of was more, with an average of 5.67, the plant height was 2.88 cm, the stem diameter was 1.44 mm, the leaf length was 11.69 mm, the leaf width was 8.54 mm, the plant leaf was large, the SPAD value was 39.23, the chlorophyll content of the leaf was higher, and the overall growth of the plant was robust without yellow leaf phenomenon.【Conclusion】LED light conditions have an impact on callus induction, proliferation culture, and strong seedling culture of A. andraeanum Fuxing in tissue culture. According to the lighting requirements at different stages of A. andraeanum tissue culture, selecting appropriate LED light quality ratio and light intensity conditions can effectively improve the callus induction rate, proliferation rate and tissue culture seedling production quality of A. andraeanum in tissue culture, and provide technical support for the efficient and standardized production of new A. andraeanum cultivar Fuxing.
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