Development of a high-performance liquid chromatography for analysis of corosolic acid in Lagerstroemia species and their hypoglycemic potentials

Abstract

Worldwide people suffer from metabolic syndrome and its complications. For these conditions, functional foods are becoming more important. We aimed to determine the amounts of corosolic acid in Lagerstroemia species and their αglucosidase inhibitory potency. In addition, we developed a new source of corosolic acid using the plant tissue culture technique. The HPLC-UV method was reliable and applicable for corosolic acid determination of the Lagerstroemia species. Although the corosolic acid standardized extract is usually prepared using L. speciosa, our results revealed that L. macrocarpa and L. loudonii contained much higher amounts of corosolic acid. In addition, the established callus culture of L. speciosa produced corosolic acid with higher content than their parental L. speciosa mature leaves. The corosolic contents in these samples were also in agreement with α-glucosidase inhibitory activity. Therefore, this method is worthy of antidiabetic standardization of Lagerstroemia derived materials.

Keywords

• lagerstroemia
• corosolic acid
• hplc
• α-glucosidase inhibition
• diabetes