BrDHC1, a Novel Putative DEAD-Box Helicase Gene, Confers Drought Tolerance in Transgenic Brassica rapa

Gangqiang Cao; Huihui Gu; Wenjing Jiang; Zhaoran Tian; Gongyao Shi; Weiwei Chen; Baoming Tian; Xiaochun Wei; Luyue Zhang; Fang Wei; Zhengqing Xie

doi:10.3390/horticulturae8080707

Horticulturae (Aug 2022)

BrDHC1, a Novel Putative DEAD-Box Helicase Gene, Confers Drought Tolerance in Transgenic Brassica rapa

Gangqiang Cao,
Huihui Gu,
Wenjing Jiang,
Zhaoran Tian,
Gongyao Shi,
Weiwei Chen,
Baoming Tian,
Xiaochun Wei,
Luyue Zhang,
Fang Wei,
Zhengqing Xie

Affiliations

Gangqiang Cao: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Huihui Gu: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Wenjing Jiang: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Zhaoran Tian: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Gongyao Shi: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Weiwei Chen: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Baoming Tian: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Xiaochun Wei: Institute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T and R Base of Zhengzhou University, Zhengzhou 450002, China
Luyue Zhang: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Fang Wei: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
Zhengqing Xie: Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China

DOI: https://doi.org/10.3390/horticulturae8080707
Journal volume & issue: Vol. 8, no. 8
p. 707

Abstract

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Drought can seriously hinder the growth of plants, resulting in reduced crop yield and quality. At present, the tolerance of DEAD-box helicases (DHC) to abiotic stresses, such as drought, high salinity, low temperature, and high temperature, has been confirmed in a variety of plants; therefore, using DEAD-box helicases to develop stress-resistant plants has great application prospects. In this study, Brassica rapa was used as a model to explore the response of the BrDHC1 gene to drought stress by creating RNA interference and overexpressing lines in B. rapa. The mechanism of BrDHC1 involved in drought resistance was revealed by the analysis of morphological characteristics, physiological indicators, and expression analysis of related stress response genes. The results showed that the overexpression of the BrDHC1 gene was more conducive to enhancing the resilience of plants under drought stress in B. rapa. Taken together, these results confirmed BrDHC1 as a newly identified DEAD-box helicase gene that could actively regulate plant growth and development under drought stress in B. rapa.

Published in Horticulturae

ISSN: 2311-7524 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: http://www.mdpi.com/journal/horticulturae

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