Synergistic effect of persica mouthwash and Iranian ethanolic extract of propolis against biofilm formation of oral pathogens (in vitro study)

Abstract

ABSTRACT Aims: Several pathogens are responsible for oral diseases and dental plaque and their main mechanism is biofilm production. Natural products are point of interest for controlling these infections. persica mouthrinse, propolis and honey are some of these products with considerable antibacterial effects. In this study, we aimed to investigate synergy effect of these products on their antibiofilm and antibacterial effect. Material and Methods: Minimal Inhibitory effect and Minimial Biofilm inhibitory concentration of persica mouthrinse, propolis, honey solely and in combination was calculated against Streptococcus mutans ATCC35668, methicillin-resistant Staphylococcus aureus ATCC 33591, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis, Enterococcus faecalis ATCC 29212 , Escherichia coli ATCC 25922. Results: Combination of persica and propolis had a better profile in biofilm’s inhibition than honey. None of herbal combinations had synergistic effect against studied bacteria; MIC of the persica mouth had the best effect against Streptococcus mutans, which causes caries. Conclusions: There was no synergistic effect of persica and propolis and the best antimicrobial effect was observed on subminimum inhibitory concentration of persica mouthwash. Findings of the present study suggest use of other combination than honey, propolis and persica for improving antimicrobial activity of these components.

Keywords

• Streptococcus mutans
• Staphylococcus aureus
• biofilm
• placa
• persica
• propóleos
• miel