Regulatory Mechanisms in Biosystems (Sep 2014)
Structure and function of the liver in conditions of chrome-isoniazid-rifampicin affection of rats after applying of sorbex
Abstract
The aim of this research was to assess the activity of marker enzymes of the liver and its biliary formation function in conditions of the affection of animals by hexavalent chromium compounds, isoniazid and rifampicin, after applying of sorbex. The experimental affection of rats of different age was carried in the conditions of combined injection of hexavalent chromium compounds (solution of potassium dichromate, 3 mg/kg), isoniazid (0.05 g/kg) and rifampicin (0.25 g/kg) during the 7th and 14th days, and sorbex enterosorbent was introduced in quantity of 150 mg/kg. The activity of marker enzymes of the liver was evaluated by the activity of alanine and aspartate aminotransferases (ALT and AST) and alkaline phosphatase (ALP). The state of biliary formation function of the liver was evaluated by the content of total bilirubin (TB) and bile acids (BA) in blood. The most significant changes in ALT activity were observed in the liver of old animals by the combined effects of the abovementioned xenobiotics – the activity of ALT was decreased by the end of the experiment by 58% compared with the animals of intact control. Using of sorbex led to decreasing in blood serum and increasing in the liver of affected animals of the different age of ALT activity throughout the experiment. AST activity in blood serum increased, and it was the highest in old animals upon chrome-isoniazid-rifampicin affection on the 14th day of the research. With the use of sorbex, there was a tendency to normalization of this index in blood serum and liver of affected animals on the 7th day from the beginning of the experiment. It was found that the largest increase in ALP took place in blood serum of immature animals by the combined effects of toxicants. In the liver of affected animals the activity of ALP decreased throughout the experiment in all age groups of animals. Maximum corrective effect on the activity of ALP was shown by the enterosorbent in the liver of mature animals on 14th day of the experiment and this index was equal to 99% compared with intact animals. During the affection of animals by toxins, we observed the increase in the content of total bilirubin and bile acids, especially in rats of immature age and mature age. After the injection of sorbex enterosorbent in the organism of animals, normalization of its indicators on the 7th day from the beginning of the experiment was established. It is proved that sorbex has normalized the activity of marker enzymes of the liver and its biliary formation function in the organism of affected animals. Therefore, it can be used as an enterosorbent in the conditions of chemical affection on the background of drug-induced hepatitis.
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