Nature Communications (Aug 2022)
Single cell spatial analysis reveals the topology of immunomodulatory purinergic signaling in glioblastoma
- Shannon Coy,
- Shu Wang,
- Sylwia A. Stopka,
- Jia-Ren Lin,
- Clarence Yapp,
- Cecily C. Ritch,
- Lisa Salhi,
- Gregory J. Baker,
- Rumana Rashid,
- Gerard Baquer,
- Michael Regan,
- Prasidda Khadka,
- Kristina A. Cole,
- Jaeho Hwang,
- Patrick Y. Wen,
- Pratiti Bandopadhayay,
- Mariarita Santi,
- Thomas De Raedt,
- Keith L. Ligon,
- Nathalie Y. R. Agar,
- Peter K. Sorger,
- Mehdi Touat,
- Sandro Santagata
Affiliations
- Shannon Coy
- Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School
- Shu Wang
- Laboratory of Systems Pharmacology, Harvard Program in Therapeutic Science
- Sylwia A. Stopka
- Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School
- Jia-Ren Lin
- Laboratory of Systems Pharmacology, Harvard Program in Therapeutic Science
- Clarence Yapp
- Laboratory of Systems Pharmacology, Harvard Program in Therapeutic Science
- Cecily C. Ritch
- Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School
- Lisa Salhi
- Sorbonne Université, Inserm, CNRS, UMR S 1127, Institut du Cerveau et de la Moelle Epinière, and AP-HP Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix
- Gregory J. Baker
- Laboratory of Systems Pharmacology, Harvard Program in Therapeutic Science
- Rumana Rashid
- Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School
- Gerard Baquer
- Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School
- Michael Regan
- Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School
- Prasidda Khadka
- Department of Pediatric Oncology, Dana-Farber Boston Children’s Cancer and Blood Disorders Center
- Kristina A. Cole
- Children’s Hospital of Philadelphia, University of Pennsylvania
- Jaeho Hwang
- Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School
- Patrick Y. Wen
- Department of Neurology, Brigham and Women’s Hospital, Harvard Medical School
- Pratiti Bandopadhayay
- Department of Pediatric Oncology, Dana-Farber Boston Children’s Cancer and Blood Disorders Center
- Mariarita Santi
- Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia
- Thomas De Raedt
- Children’s Hospital of Philadelphia, University of Pennsylvania
- Keith L. Ligon
- Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School
- Nathalie Y. R. Agar
- Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School
- Peter K. Sorger
- Laboratory of Systems Pharmacology, Harvard Program in Therapeutic Science
- Mehdi Touat
- Sorbonne Université, Inserm, CNRS, UMR S 1127, Institut du Cerveau et de la Moelle Epinière, and AP-HP Hôpitaux Universitaires La Pitié Salpêtrière - Charles Foix
- Sandro Santagata
- Department of Pathology, Brigham and Women’s Hospital, Harvard Medical School
- DOI
- https://doi.org/10.1038/s41467-022-32430-w
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 24
Abstract
The components of the glioma immune microenvironment and their roles in promoting tumourigenesis remain poorly understood. Here, the use of single-cell RNA sequencing and multiplexed tissue-imaging in adult and pediatric high-grade gliomas reveals the activity and spatial organization of the immunomodulatory purinergic signaling pathway.
