Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene

Haiyan Zhang; Chen Liu; Yanyan Ma; Lin Lin; Yuqiang Lv; Min Gao; Zhongtao Gai; Yi Liu

Stem Cell Research (May 2021)

Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene

Haiyan Zhang,
Chen Liu,
Yanyan Ma,
Lin Lin,
Yuqiang Lv,
Min Gao,
Zhongtao Gai,
Yi Liu

Affiliations

Haiyan Zhang: Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Chen Liu: Neonatal Department, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Yanyan Ma: Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Lin Lin: Neonatal Department, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Yuqiang Lv: Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Min Gao: Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Zhongtao Gai: Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China
Yi Liu: Pediatric Research Institute, Qilu Children’s Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250022, China; Corresponding author.

Journal volume & issue: Vol. 53
p. 102331

Abstract

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Here we describe the generation of induced pluripotent stem cells (iPSCs) from a patient diagnosed as hereditary tyrosinemia type I (HT1) caused by FAH gene mutation. Induced pluripotent stem cells (iPSCs) were developed using non-integrating episomal vectors containing OCT4, SOX2, KLF4, BCL-XL and MYC. The established iPSC line (SDQLCHi026-A) displayed pluripotent cell morphology, high expression levels of pluripotency markers, differentiation potential in vitro, normal karyotype, and remaining the original FAH gene mutation.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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