Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells

Santosh Adhikari; Joe Moscatelli; Elizabeth M. Smith; Chiranjib Banerjee; Elias M. Puchner

doi:10.1038/s41467-019-11384-6

Nature Communications (Jul 2019)

Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells

Santosh Adhikari,
Joe Moscatelli,
Elizabeth M. Smith,
Chiranjib Banerjee,
Elias M. Puchner

Affiliations

Santosh Adhikari: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Joe Moscatelli: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Elizabeth M. Smith: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Chiranjib Banerjee: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)
Elias M. Puchner: School of Physics and Astronomy University of Minnesota, Twin Cities Physics and Nanotechnology (PAN)

DOI: https://doi.org/10.1038/s41467-019-11384-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Single-molecule localization microscopy (SMLM) requires the use of fluorophores with specific sets of properties. Here the authors employ conventional BODIPY dyes as SMLM fluorophores by making use of rarely reported red-shifted ground state BODIPY dimers to image fatty acids, lipid droplets and lysosomes at single-molecule resolution.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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