The mechanism of DNA unwinding by the eukaryotic replicative helicase

Daniel R. Burnham; Hazal B. Kose; Rebecca B. Hoyle; Hasan Yardimci

doi:10.1038/s41467-019-09896-2

Nature Communications (May 2019)

The mechanism of DNA unwinding by the eukaryotic replicative helicase

Daniel R. Burnham,
Hazal B. Kose,
Rebecca B. Hoyle,
Hasan Yardimci

Affiliations

Daniel R. Burnham: The Francis Crick Institute
Hazal B. Kose: The Francis Crick Institute
Rebecca B. Hoyle: School of Mathematical Sciences, University of Southampton
Hasan Yardimci: The Francis Crick Institute

DOI: https://doi.org/10.1038/s41467-019-09896-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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How the eukaryotic helicase unzips DNA during replication is not well understood. By measuring the real-time motion of purified CMG unwinding DNA with magnetic tweezers, the authors reveal the dynamics where isolated CMG unwinds via a biased random walk with proclivity to pause.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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