Frontiers in Cellular and Infection Microbiology (Oct 2023)

Isolation and identification of Tete virus group (Peribunyaviridae: Orthobunyavirus) from Culicoides biting midges collected in Lichuan County, China

  • Qikai Yin,
  • Rui Cheng,
  • Rui Cheng,
  • Rui Cheng,
  • Xiuyan Xu,
  • Xiuyan Xu,
  • Ziqian Xu,
  • Jing Wang,
  • Jing Wang,
  • Shihong Fu,
  • Hongbin Xu,
  • Shaozai Zhang,
  • Ying He,
  • Fan Li,
  • Songtao Xu,
  • Xiaoqing Lu,
  • Huanyu Wang,
  • Bin Wang,
  • Guodong Liang

DOI
https://doi.org/10.3389/fcimb.2023.1193184
Journal volume & issue
Vol. 13

Abstract

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In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 cells, and the virus titer was 1×105.6 pfu/mL. After inoculation with the virus, suckling mice developed disease and died. The nucleotide and amino sequence analysis showed that the JXLC1806-2 virus genome was composed of S, M and L segments. Phylogenetic analysis showed that the S, M and L genes of JXLC1806-2 virus belonged to the Tete serogroup, Orthobunyavirus, but formed an independent evolutionary branch from the other members of the Tete serogroup. The results showed that the JXLC1806-2 virus, which was named as Lichuan virus, is a new member of Tete serogroup, and this is the first time that a Tete serogroup virus has been isolated in China.

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