International Journal of Molecular Sciences (May 2023)
Conjugated Dienoic Acid Peroxides as Substrates in <i>Chaetopterus</i> Bioluminescence System
- Renata I. Zagitova,
- Konstantin V. Purtov,
- Aleksandr S. Shcheglov,
- Konstantin S. Mineev,
- Maxim A. Dubinnyi,
- Ivan N. Myasnyanko,
- Olga A. Belozerova,
- Vera G. Pakhomova,
- Valentin N. Petushkov,
- Natalia S. Rodionova,
- Vladislav A. Lushpa,
- Elena B. Guglya,
- Sergey Kovalchuk,
- Valeri B. Kozhemyako,
- Jeremy D. Mirza,
- Anderson G. Oliveira,
- Ilia V. Yampolsky,
- Zinaida M. Kaskova,
- Aleksandra S. Tsarkova
Affiliations
- Renata I. Zagitova
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Konstantin V. Purtov
- Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia
- Aleksandr S. Shcheglov
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Konstantin S. Mineev
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Maxim A. Dubinnyi
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Ivan N. Myasnyanko
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Olga A. Belozerova
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Vera G. Pakhomova
- Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50, 660036 Krasnoyarsk, Russia
- Valentin N. Petushkov
- Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia
- Natalia S. Rodionova
- Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia
- Vladislav A. Lushpa
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Elena B. Guglya
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Sergey Kovalchuk
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Valeri B. Kozhemyako
- Central Research Laboratory, Pacific State Medical University, Ostryakova 2, 690002 Vladivostok, Russia
- Jeremy D. Mirza
- School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
- Anderson G. Oliveira
- Department of Chemistry and Biochemistry, Yeshiva University, 245 Lexington Ave, New York, NY 10016, USA
- Ilia V. Yampolsky
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Zinaida M. Kaskova
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- Aleksandra S. Tsarkova
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia
- DOI
- https://doi.org/10.3390/ijms24119466
- Journal volume & issue
-
Vol. 24,
no. 11
p. 9466
Abstract
Biochemistry of bioluminescence of the marine parchment tubeworm Chaetopterus has been in research focus for over a century; however, the results obtained by various groups contradict each other. Here, we report the isolation and structural elucidation of three compounds from Chaetomorpha linum algae, which demonstrate bioluminescence activity with Chaetopterus luciferase in the presence of Fe2+ ions. These compounds are derivatives of polyunsaturated fatty acid peroxides. We have also obtained their structural analogues and demonstrated their activity in the bioluminescence reaction, thus confirming the broad substrate specificity of the luciferase.
Keywords
