Explant Methodology for Analyzing Neuroblast Migration

Kirsty Dixon; Alisa Turbic; Ann Turnley; Daniel Liebl

doi:10.21769/BioProtoc.2249

Bio-Protocol (May 2017)

Explant Methodology for Analyzing Neuroblast Migration

Kirsty Dixon,
Alisa Turbic,
Ann Turnley,
Daniel Liebl

Affiliations

Kirsty Dixon: Department of Transplant Surgery, Virginia Commonwealth University, Richmond, USA
Alisa Turbic: Department of Epidemiology and Preventive Medicine, Monsah University, Melbourne, Australia
Ann Turnley: Department of Anatomy and Neuroscience, The University of Melbourne, Parkville, Australia
Daniel Liebl: The Miami Project to Cure Paralysis and Department of Neurological Surgery, University of Miami, Miami, USA

DOI: https://doi.org/10.21769/BioProtoc.2249
Journal volume & issue: Vol. 7, no. 9

Abstract

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The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we describe the isolation of SVZ-derived RMS explants from the neonatal mouse brain, and the conditions required to culture and evaluate their migration.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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