Post-supplementation nutrient profiles of proteins, amino acids and DNA in Macrobrachium rosenbergii PL-15

Abstract

Abstract Background The study was indented to obtain a protein profile pattern using SDS-PAGE, an amino acid profile pattern using high-performance thin-layer chromatography, and a DNA profile pattern using agarose gel electrophoresis in Macrobrachium rosenbergii (post larva (PL)-15) after oral supplementation with three herbs, namely, Allium sativum, Curcuma longa, and Zingiber officinale. Each herb was individually supplemented along with a basal feed mix at 1%, 3%, and 5% for 90 days to the corresponding experimental groups. Control groups were offered only the basal feed mix (0% supplementation). Proteins were extracted and electrophoresed. Amino acids were extracted and chromatographed. DNA was isolated and electrophoresed. Results The study revealed the superlative performance of Z. officinale, which when supplemented at the 5% level, promoted synthesis of 66-kDa, 41-kDa, 15-kDa, and 13-kDa proteins; synthesis of certain essential amino acids including lysine and methionine; stabilized the integrity of DNA. Conclusion The presence of nutraceuticals such as alkaloids, flavonoids, pigments, phenolics, terpenoids, steroids, and essential oils in the supplemented herbs is the causative factor for increased nutritional quality through higher rates of transcription and antioxidation, in addition to the bioenhancer effect, which promoted protein intake.

Keywords

• Aquaculture
• Aqua feed
• Sustainable aquaculture
• Macrobrachium rosenbergii
• Prebiotics
• Nutraceuticals