Photometric Assays for Chloroplast Movement Responses to Blue Light

Halina Gabryś; Agnieszka Banaś; Pawel Hermanowicz; Weronika Krzeszowiec; Sebastian Leśniewski; Justyna Łabuz; Olga Sztatelman

doi:10.21769/BioProtoc.2310

Bio-Protocol (Jun 2017)

Photometric Assays for Chloroplast Movement Responses to Blue Light

Halina Gabryś,
Agnieszka Banaś,
Pawel Hermanowicz,
Weronika Krzeszowiec,
Sebastian Leśniewski,
Justyna Łabuz,
Olga Sztatelman

Affiliations

Halina Gabryś: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
Agnieszka Banaś: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
Pawel Hermanowicz: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
Weronika Krzeszowiec: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
Sebastian Leśniewski: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
Justyna Łabuz: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandMalopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, Krakow, Poland
Olga Sztatelman: Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, Warsaw, Poland

DOI: https://doi.org/10.21769/BioProtoc.2310
Journal volume & issue: Vol. 7, no. 11

Abstract

Read online

Assessment of chloroplast movements by measuring changes in leaf transmittance is discussed, with special reference to the conditions necessary for reliable estimation of blue light–activated chloroplast responses.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

About the journal