Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Shane M. Hickey; Ben Ung; Christie Bader; Robert Brooks; Joanna Lazniewska; Ian R. D. Johnson; Alexandra Sorvina; Jessica Logan; Carmela Martini; Courtney R. Moore; Litsa Karageorgos; Martin J. Sweetman; Douglas A. Brooks

doi:10.3390/cells11010035

Cells (Dec 2021)

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Shane M. Hickey,
Ben Ung,
Christie Bader,
Robert Brooks,
Joanna Lazniewska,
Ian R. D. Johnson,
Alexandra Sorvina,
Jessica Logan,
Carmela Martini,
Courtney R. Moore,
Litsa Karageorgos,
Martin J. Sweetman,
Douglas A. Brooks

Affiliations

Shane M. Hickey: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Ben Ung: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Christie Bader: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Robert Brooks: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Joanna Lazniewska: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Ian R. D. Johnson: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Alexandra Sorvina: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Jessica Logan: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Carmela Martini: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Courtney R. Moore: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Litsa Karageorgos: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Martin J. Sweetman: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia
Douglas A. Brooks: Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia

DOI: https://doi.org/10.3390/cells11010035
Journal volume & issue: Vol. 11, no. 1
p. 35

Abstract

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Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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Abstract

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