In vivo cell type-specific CRISPR knockdown of dopamine beta hydroxylase reduces locus coeruleus evoked wakefulness

Hiroshi Yamaguchi; F. Woodward Hopf; Shi-Bin Li; Luis de Lecea

doi:10.1038/s41467-018-07566-3

Nature Communications (Dec 2018)

In vivo cell type-specific CRISPR knockdown of dopamine beta hydroxylase reduces locus coeruleus evoked wakefulness

Hiroshi Yamaguchi,
F. Woodward Hopf,
Shi-Bin Li,
Luis de Lecea

Affiliations

Hiroshi Yamaguchi: Department of Psychiatry and Behavioral Sciences, Stanford University
F. Woodward Hopf: Alcohol and Addiction Research Group, Department of Neurology, University of California at San Francisco
Shi-Bin Li: Department of Psychiatry and Behavioral Sciences, Stanford University
Luis de Lecea: Department of Psychiatry and Behavioral Sciences, Stanford University

DOI: https://doi.org/10.1038/s41467-018-07566-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 8

Abstract

Read online

Neurons of the locus coeruleus (LC) are implicated in attention and arousal. Here the authors use CRISPR/Cas9 to disrupt dopamine beta hydroxylase in LC neurons and see this reduces LC-evoked sleep-to-wake transitions and wake length.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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