Protocol for producing hyperpolarized 13C-bicarbonate for clinical MRI of extracellular pH in aggressive tumors

Changhua Mu; Xiaoxi Liu; Andrew Riselli; James Slater; Evelyn Escobar; Duy Dang; Scott Drapeau; Romelyn Delos Santos; Stacy Andosca; Hao Nguyen; Peder E.Z. Larson; Robert Bok; Daniel B. Vigneron; John Kurhanewicz; David M. Wilson; Robert R. Flavell

STAR Protocols (Sep 2024)

Protocol for producing hyperpolarized 13C-bicarbonate for clinical MRI of extracellular pH in aggressive tumors

Changhua Mu,
Xiaoxi Liu,
Andrew Riselli,
James Slater,
Evelyn Escobar,
Duy Dang,
Scott Drapeau,
Romelyn Delos Santos,
Stacy Andosca,
Hao Nguyen,
Peder E.Z. Larson,
Robert Bok,
Daniel B. Vigneron,
John Kurhanewicz,
David M. Wilson,
Robert R. Flavell

Affiliations

Changhua Mu: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA; Corresponding author
Xiaoxi Liu: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Andrew Riselli: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
James Slater: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Evelyn Escobar: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Duy Dang: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Scott Drapeau: Makers Lab, Library, University of California, San Francisco, San Francisco, CA 94143, USA
Romelyn Delos Santos: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Stacy Andosca: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Hao Nguyen: Department of Urology, University of California, San Francisco, San Francisco, CA 94143, USA
Peder E.Z. Larson: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Robert Bok: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Daniel B. Vigneron: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
John Kurhanewicz: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
David M. Wilson: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA
Robert R. Flavell: Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA

Journal volume & issue: Vol. 5, no. 3
p. 103091

Abstract

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Summary: Tumor acidosis is one of the hallmarks indicating the initiation and progression of various cancers. Here, we present a protocol for preparing a hyperpolarized (HP) 13C-bicarbonate tissue pH MRI imaging contrast agent to detect aggressive tumors. We describe the steps for the formulation and polarization of a precursor molecule 13C-glycerol carbonate (13C-GLC), the post-dissolution reaction, and converting HP 13C-GLC to an injectable HP 13C-bicarbonate solution. We then detail procedures for MRI data acquisition to generate tumor pH maps for assessing tumor aggressiveness.For complete details on the use and execution of this protocol, please refer to Mu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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