Characterization of Pratylenchus crenatus and P. neglectus (Nematoda: Pratylenchidae) associated with wheat crop

Abstract

The distribution of Pratylenchus species associated with wheat crops was investigated in Bohemian region of the Czech Republic. In total twelve localities were sampled. The populations were identified based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2/D3 region and 18S gene. Pratylenchus crenatus was present in two localities and P. neglectus in five localities. At one locality both species were detected. Sequence analysis of 18S and D2/D3 region of three populations of P. crenatus reveal no variation while five populations of P. neglectus differ by 0 to 0.14 % (18S) and 0.17 to 0.50 % (D2/D3).

Keywords

• pratylenchus crenatus
• pratylenchus neglectus
• pcr
• ribosomal dna
• sequencing
• nematode