Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activity against Multidrug-Resistant Bacteria and Wound Healing Efficacy Using a Murine Model

Vajravathi Lakkim; Madhava C. Reddy; Roja Rani Pallavali; Kakarla Raghava Reddy; Ch Venkata Reddy; Inamuddin; Anwar L. Bilgrami; Dakshayani Lomada

doi:10.3390/antibiotics9120902

Antibiotics (Dec 2020)

Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activity against Multidrug-Resistant Bacteria and Wound Healing Efficacy Using a Murine Model

Vajravathi Lakkim,
Madhava C. Reddy,
Roja Rani Pallavali,
Kakarla Raghava Reddy,
Ch Venkata Reddy,
Inamuddin,
Anwar L. Bilgrami,
Dakshayani Lomada

Affiliations

Vajravathi Lakkim: Department of Genetics and Genomics, Yogi Vemana University, Kadapa, AP 516005, India
Madhava C. Reddy: Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa, AP 516005, India
Roja Rani Pallavali: Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa, AP 516005, India
Kakarla Raghava Reddy: School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW 2006, Australia
Ch Venkata Reddy: School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749, Korea
Inamuddin: Advanced Functional Materials Laboratory, Department of Applied Chemistry, Zakir Husain College of Engineering and Technology, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, UP 202002, India
Anwar L. Bilgrami: Deanship of Scientific Research, King Abdulaziz University, Jeddah 80216, Saudi Arabia
Dakshayani Lomada: Department of Genetics and Genomics, Yogi Vemana University, Kadapa, AP 516005, India

DOI: https://doi.org/10.3390/antibiotics9120902
Journal volume & issue: Vol. 9, no. 12
p. 902

Abstract

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Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using Catharanthus roseus and Azadirachta indica extracts, were characterized using UV–Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Silver nanoparticles (Ag NPs) synthesized from leaf extracts of C. roseus and A. indica effectively inhibited the growth of multidrug-resistant (MDR) bacteria isolated from patients with septic wound infections. The maximum bacteriolytic activity of the green-synthesized Ag NPs of C. roseus and A. indica against the MDR bacterium K. Pneumoniae was shown by a zone of inhibition of 19 and 16 mm, respectively. C. roseus Ag NPs exhibited more bacteriolytic activity than A. indica Ag NPs in terms of the zone of inhibition. Moreover, these particles were effective in healing wounds in BALB/c mice. Ag NPs of C. roseus and A. indica enhanced wound healing by 94% ± 1% and 87% ± 1%, respectively. Our data suggest that Ag NPs from C. roseus and A. indicia ameliorate excision wounds, and wound healing could be due to their effective antimicrobial activity against MDR bacteria. Hence, these Ag NPs could be potential therapeutic agents for the treatment of wounds.

Published in Antibiotics

ISSN: 2079-6382 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antibiotics

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