Regulation of plasma lipid homeostasis by hepatic lipoprotein lipase in adult mice[S]

Gan Liu; Jun-Nan Xu; Dong Liu; Qingli Ding; Meng-Na Liu; Rong Chen; Mengdi Fan; Ye Zhang; Chao Zheng; Da-Jin Zou; Jianxin Lyu; Weiping J. Zhang

Journal of Lipid Research (Jul 2016)

Regulation of plasma lipid homeostasis by hepatic lipoprotein lipase in adult mice[S]

Gan Liu,
Jun-Nan Xu,
Dong Liu,
Qingli Ding,
Meng-Na Liu,
Rong Chen,
Mengdi Fan,
Ye Zhang,
Chao Zheng,
Da-Jin Zou,
Jianxin Lyu,
Weiping J. Zhang

Affiliations

Gan Liu: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China; Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China
Jun-Nan Xu: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China
Dong Liu: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China; Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenchou Medical University School of Laboratory Medicine and Life Sciences, Wenchou, Zhejiang 325035, China
Qingli Ding: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China
Meng-Na Liu: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China; Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenchou Medical University School of Laboratory Medicine and Life Sciences, Wenchou, Zhejiang 325035, China
Rong Chen: Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China; Department of Endocrinology, Changhai Hospital, Shanghai 200433, China
Mengdi Fan: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China; Department of Endocrinology, Second Affiliated Hospital, Wenchou Medical University, Wenchou, Zhejiang 325000, China
Ye Zhang: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China; Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China
Chao Zheng: Department of Endocrinology, Second Affiliated Hospital, Wenchou Medical University, Wenchou, Zhejiang 325000, China
Da-Jin Zou: Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China; Department of Endocrinology, Changhai Hospital, Shanghai 200433, China
Jianxin Lyu: To whom correspondence should be addressed. (W.J.Z.); (J.L.); Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenchou Medical University School of Laboratory Medicine and Life Sciences, Wenchou, Zhejiang 325035, China; To whom correspondence should be addressed. (W.J.Z.); (J.L.)
Weiping J. Zhang: To whom correspondence should be addressed. (W.J.Z.); (J.L.); Department of Pathophysiology Second Military Medical University, Shanghai 200433, China; Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China; To whom correspondence should be addressed. (W.J.Z.); (J.L.)

Journal volume & issue: Vol. 57, no. 7
pp. 1155 – 1161

Abstract

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LPL is a pivotal rate-limiting enzyme to catalyze the hydrolysis of TG in circulation, and plays a critical role in regulating lipid metabolism. However, little attention has been paid to LPL in the adult liver due to its relatively low expression. Here we show that endogenous hepatic LPL plays an important physiological role in plasma lipid homeostasis in adult mice. We generated a mouse model with the Lpl gene specifically ablated in hepatocytes with the Cre/LoxP approach, and found that specific deletion of hepatic Lpl resulted in a significant decrease in plasma LPL contents and activity. As a result, the postprandial TG clearance was markedly impaired, and plasma TG and cholesterol levels were significantly elevated. However, deficiency of hepatic Lpl did not change the liver TG and cholesterol contents or glucose homeostasis. Taken together, our study reveals that hepatic LPL is involved in the regulation of plasma LPL activity and lipid homeostasis.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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