Generation of eight human induced pluripotent stem cell lines from Parkinson's disease patients carrying familial mutations

Muwan Chen; Muyesier Maimaitili; Susanne Hvolbøl Buchholdt; Uffe Birk Jensen; Fabia Febbraro; Mark Denham

Stem Cell Research (Jan 2020)

Generation of eight human induced pluripotent stem cell lines from Parkinson's disease patients carrying familial mutations

Muwan Chen,
Muyesier Maimaitili,
Susanne Hvolbøl Buchholdt,
Uffe Birk Jensen,
Fabia Febbraro,
Mark Denham

Affiliations

Muwan Chen: Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Denmark; Department of Biomedicine, Aarhus University, Denmark
Muyesier Maimaitili: Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Denmark; Department of Biomedicine, Aarhus University, Denmark
Susanne Hvolbøl Buchholdt: Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Denmark; Department of Biomedicine, Aarhus University, Denmark
Uffe Birk Jensen: Department of Biomedicine, Aarhus University, Denmark; Department for Clinical Genetics, Aarhus University Hospital, Denmark
Fabia Febbraro: Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Denmark; Department of Health Science and Technology, Aalborg University, Denmark
Mark Denham: Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic EMBL Partnership for Molecular Medicine, Aarhus University, Denmark; Department of Biomedicine, Aarhus University, Denmark; Corresponding author.

Journal volume & issue: Vol. 42

Abstract

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We generated eight induced pluripotent stem cell (iPSC) lines from Parkinson's disease (PD) patients with different familial mutations using non-integrating episomal plasmids. All iPSC lines have a normal karyotype, express pluripotent genes including POU5F1, NANOG, and show alkaline phosphatase activity, as well as the ability to differentiate into all three germ layers. These PD iPSC lines can be used for disease modeling to identify PD mechanisms and for the development or stratification of new drugs.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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